What happens when you boil a leaf?
Boiling the leaf damages the membranes of the cells allowing the pigments to be leached out of the cells. After leaching, the iodine solution added will combine with any starch in the cells producing a dark purple to black pigment in the leaf.
Why is the leaf soaked in boiling water at first?
This procedure kills a leaf, disrupts the cell membranes and softens the cuticle and cell walls. This makes it possible to extract the chlorophyll with hot ethanol and also allows the iodine solution to penetrate the cells and react with any starch present.
What is the purpose of boiling the leaf in methylated spirit?
Because Methylated spirit is an alcohol & when we boil the leaf in alcohol we are testing it for starch as this would remove the chlorophyll from the green leaf . Deolourish leaf is than treated with iodine & a blue black color is obtained which confirms the presence of starch in the green portion of leaf.
Why do we need to Decolourise the leaf?
we need to decolourise the leaf so that when we add starch solution the stains can be observed carefully. we decolourise the leaf by double boiling it in alcohol.
Why must the leaf be freshly picked?
Freshly picked raw leaves in particular are teeming with activity and vitality. Kirlian photography can measure this vitality, or life force, and it reduces when an individual plant or animal becomes sick or very old. When you eat fresh raw leaves you are taking that vitality into you.
Why do we need to boil the leaf in water before testing it for starch?
We boil the leaf in alcohol when we are checking it for starch to eradicate chlorophyll, which is the green pigment present in leaves. During the starch test, we observe the colour change of leaf from brown to blue when iodine is put on the leaf as iodine reacts with the starch present in the leaf.
Why is it necessary to boil the ethanol tube in the water bath?
the tube is immersed in water bath as it contains alcohol and alcohol is easily flammable. by heating the alcohol directly ,it will catch fire. it is immersed in water bath so that alcohol doesn’t catch fire.
Why must the leaf be rinsed in water?
Explain the significance of rinsing the leaf in water.
The leaf was rinsed in water to rehydrate it. Iodine solution is an aqueous solution of iodine/potassium iodie – potassium tri-iodide; water is needed inside the leaf to enable penetration by diffusion.
What are the major precautions you need to take in this experiment leaf boiling in methylated spirit?
1. Wear safety goggles. 2. Keep ethanol away from Bunsen burner; ethanol is 3.
Why is the leaf placed in warm water after boiling it in methylated spirit?
Drop the leaf in boiling water to kill it. Put the leaf in methylated spirits to remove the green substance called chlorophyll. Place the leaf in an iodine solution. The part of the leaf not covered by the silver paper turns blue black colour.
Why are you thinking that photosynthesis is important?
Photosynthesis is important to living organisms because it is the number one source of oxygen in the atmosphere. Without photosynthesis, the carbon cycle could not occur, oxygen-requiring life would not survive and plants would die. … Without photosynthesis there would be little to no oxygen on the planet.